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Trace determination of aliphatic amines using HPLC with chemiluminescence excitation and photon counting
Summary
Type:
HPLC
Application #:
101290
Catalog#:
720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection:
chemiluminescence
Conditions:
eluent: acetonitrile - 0.05M Tris-HCl buffer pH 7.7 (75:25, v/v),
flow rate 2ml/min
Authors:
Mellbin G
Source:
J Chromatogr 312 (1984) 203 - 210
Details
Legend: left figure: chromatogram of NBD derivatives (injected volume 20µl, sample concentration 1.9 · 10-7M) right figure: chromatogram of Dns-amine derivatives (injected volume 20µl, sample concentratio
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